Biosafety Cabinet Levels

Richmond jy mckinney rw.

Biosafety cabinet levels.

Summary of biosafety levels and infections agents 4 a guide to biosafety biological safety cabinets biohazards and biosafety levels. The four biosafety levels were developed to protect against a world of select agents. A biosafety level bsl or pathogen protection level is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. Preferably this is a class ii biosafety cabinet or equivalent containment device for work with agents.

Department of health and human services hhs publication cdc 99 8395. The biosafety levels range from bsl 1 to bsl 4. Laboratory biosafety manual third edition world health organization who 2004. These agents include bacteria fungi parasites prions rickettsial agents and viruses the latter being probably the largest and most important group.

A guide to biosafety biological safety cabinets. Class ii type a2 or type c1 biosafety cabinets are suitable and do not require external ventilation. You will learn about each level on the following screens. Information about the biosafety level requirements for handling sar cov 2 covid 19 coronavirus can be found here.

Class ii type b2 which must be externally vented by design are. The levels of containment range from the lowest biosafety level 1 bsl 1 to the highest at level 4 bsl 4. References and further reading. Containment requirements are subject to modification by the ibc at its discretion depending on the circumstances presented by a specific project.

The class iii cabinet may be connected to a double door autoclave used to decontaminate all materials entering or exiting the cabinet. In the united states the centers for disease control and prevention cdc have specified these levels. Every microbiology laboratory regardless of biosafety level follows standard microbiological practices. The thermo scientific 1300 series a2 bsc meets nsf ansi 49 for a class ii type a2 biosafety cabinet.

The ibc uses the biosafety levels recommended by the cdc and nih as the usual standards of containment to be set for work with a given biohazardous material. Each biosafety level builds on the controls of the level before it. A biosafety cabinet bsc also called a biological safety cabinet or microbiological safety cabinet is an enclosed ventilated laboratory workspace for safely working with materials contaminated with or potentially contaminated with pathogens requiring a defined biosafety level several different types of bsc exist differentiated by the degree of biocontainment required.